TY - BOOK AU - Raghava Ram,N.V. AU - Nabors,M.W. TI - Cytokinin Mediated Long-term, High-frequency Plant Regeneration in Rice Tissue Cultures KW - ORYZA SATIVA L KW - RICE KW - CYTOKININ KW - REGENERATION KW - TISSUE CULTURES N2 - In Mahsuri rice callus cultures grown in light, the total amount of callus and the percentage of callus volume composed of embryogenic cells could be increased if 0.1-0.Smg·I-1 K was added to medium containing 0.5 or 1.0 mg .1-1 2,4-D. Embryogenic regions could be proliferated and maintained on the same media. E callus on initiation medium gradually produced embryoid-like structures and finally plantlets. This process could be hastened by removing E callus to a regeneration medium containing low concentrations of IAA and BAP or K. If the E callus produced by one seed were proliferated for five four-week passages on 0.5 mg .1-1 2,4-D and 0.5 mg .1-1 K and transferred to regeneration medium, 7,318 plants could be produced from 184 g of E callus UR - https://drive.google.com/file/d/1M12vrADUh0fj-ljUpgeJcD0cVCt0k283/view?usp=drivesdk ER -