TY  - BOOK
AU  - Tsugita,A.
AU  - Kawakami,T.
AU  - Uchiyama,T.
AU  - Kamo,M.
AU  - Miyatake,M.
AU  - Nozu,N.
TI  - Separation and characterization of rice proteins
N2  - Rice proteins from nine tissues and one organelle (leaf, chloroplast, stem, root, germ, dark germinated seedling, seed, bran, chaff and callus)were isolated and then separated by two-dimensional gel electrophoresis (2-DE). The protein spots were characterized according to molecular weight, isoelectric point and partial amino-terminal sequence. Electrophoresis was carried out by isoelectric focusing (IEF), nonequilibrium pH gradient electrophoresis (NEPHGE)and immobilized pH gradient (IPG)in the first dimension, and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)in the second dimension. With the aid of nine marker proteins, the patterns of IEF, NEPHGE and IPG 2-DE gels were graphically combined by computer into a single synthetic image for each tissue, respectively, and these images for the nine tissues and one organelle were again combined into a single 2-DE image for the integrated rice protein spots. The rice 2-DE gel image resolved 4892 proteins. About 3
UR  - https://drive.google.com/file/d/1Hw_dedTqIhWcC637mliYwKLGwtgqxMZ1/view?usp=drivesdk
ER  -