TY - BOOK AU - Lichter,Robert TI - Induction of haploid plants from isolated pollen of Brassica napus KW - BRASSICA NAPUS KW - COLD TREATMENT KW - ISOLATED MICROSPORES KW - EMBRYO IDS KW - HAPLOID PLANTS N2 - Embryoids and plants were obtained from ab initio culture of isolated microspores of Brassica napus (rape). The best results were obtained when the buds were incubated at 4 °C for several days before isolating the pollen. The culture medium contained 0.5 mg 1-1 1-naphthaleneacetic acid and 0.05 mg 1-1 6-benzylaminopurine as well as liquid potato extract. Good yields of embryoids were obtained with relatively high concentrations of sucrose in the medium UR - https://drive.google.com/file/d/1c68UBVXShHh9GQ5kBgxMFii8FHu3NPyP/view?usp=drivesdk ER -