Chloroplast DNA from tobacco leaves 

 -  Science, 153(3741), p.1271-1273, 1966 .
<br/><br/> DNA from tobacco leaf chloroplasts was isolated as a single component with a buoyant density in CsCl of 1.702 compared to 1.697 for nuclear DNA. 5-Methylcytosine is present in nuclear DNA but absent in chloroplast DNA. Chloroplast DNA, with a guanine-cytosine content of 43 percent, has a melting temperature of 86°C and renatures completely on slow cooling, whereas nuclear DNA (melting temperature, 84°C; guanine-cytosine content, 40 percent)does not renature. About 9 percent of the total DNA in tobacco leaves is chloroplast DNA representing about 4.7 × 10-15 gram of DNA per chloroplast with a molecular weight of approximately 4 × 10 7 
<br/><br/><label>Subjects--Topical Terms: </label><br/>DNA<br/>TOBACCO