TY  - BOOK
AU  - Jiang,W.
AU  - Liang,P.
AU  - Wang,B.
AU  - Fang,J.
AU  - Lang,J.
AU  - Tian,G.
AU  - Jiang,J.
AU  - Jiang,J.
TI  - Optimized DNA extraction and metagenomic sequencing of airborne microbial communities
KW  - AIR POLLUTION
KW  - AIRBORNE MICROORGANISM
KW  - ARTICLE
KW  - CONTROLLED STUDY
KW  - DNA BINDING
KW  - DNA DETERMINATION
KW  - DNA EXTRACTION
KW  - DNA LIBRARY
KW  - DNA SEQUENCE
KW  - GENOME ANALYSIS
KW  - METAGENOMICS
KW  - MICROBIAL COMMUNITY
KW  - NONHUMAN
KW  - PARTICULATE MATTER
KW  - PRIORITY JOURNAL
KW  - SEQUENCE ANALYSIS
KW  - BIOCHEMISTRY
KW  - CHINA
KW  - DEVICES
KW  - ENVIRONMENTAL MONITORING
KW  - EQUIPMENT DESIGN
KW  - GENETICS
KW  - ISOLATION AND PURIFICATION
KW  - METAGENOME
KW  - MICROBIAL CONSORTIUM
KW  - MICROBIOLOGY
KW  - PROCEDURES
KW  - QUALITY CONTROL
KW  - WORKFLOW
N2  - Metagenomic sequencing has been widely used for the study of microbial communities from various environments such as soil, ocean, sediment and fresh water. Nonetheless, metagenomic sequencing of microbial communities in the air remains technically challenging, partly owing to the limited mass of collectable atmospheric particulate matter and the low biological content it contains. Here we present an optimized protocol for extracting up to tens of nanograms of airborne microbial genomic DNA from collected particulate matter. With an improved sequencing library preparation protocol, this quantity is sufficient for downstream applications, such as metagenomic sequencing for sampling various genes from the airborne microbial community. The described protocol takes ~ 12 h of bench time over 2-3 d, and it can be performed with standard molecular biology equipment in the laboratory. A modified version of this protocol may also be used for genomic DNA extraction from other environmental samples of limited mass or low biological content
UR  - https://drive.google.com/file/d/1gbnvTYtocwx4ee4aF3ZyjdzvqgPOnvjV/view?usp=drivesdk
ER  -