TY  - BOOK
AU  - Trievel,R.C.
AU  - Flynn,E.M.
AU  - Houtz,R.L.
AU  - Hurley,J.H.
TI  - Mechanism of multiple lysine methylation by the SET domain enzyme Rubisco LSMT
KW  - EPSILON N METHYLLYSINE
KW  - HISTONE H3
KW  - HISTONE H4
KW  - HOLOENZYME
KW  - LARGE SUBUNIT METHYLTRANSFERASE
KW  - LYSINE
KW  - LYSINE DERIVATIVE
KW  - METHYL GROUP
KW  - PROTEIN
KW  - PROTEIN METHYLTRANSFERASE
KW  - RIBULOSEBISPHOSPHATE CARBOXYLASE
KW  - S ADENOSYLHOMOCYSTEINE
KW  - S ADENOSYLMETHIONINE
KW  - SET DOMAIN PROTEIN
KW  - UNCLASSIFIED DRUG
N2  - SET domain protein methyltransferases catalyze the transfer of methyl groups from the cofactor S-adenosylmethionine (AdoMet)to specific lysine residues of protein substrates, such as the N-terminal tails of histones H3 and H4 and the large subunit of the Rubisco holoenzyme complex. The crystal structures of pea Rubisco large subunit methyltransferase (LSMT)in ternary complexes with either lysine or e-N-methyllysine (MeLys)and the product S-adenosylhomocysteine (AdoHcy)were determined to resolutions of 2.65 and 2.55 Å, respectively. The ?-methyl group of MeLys is bound to the enzyme via carbon-oxygen hydrogen bonds that play a key role in catalysis. The methyl donor and acceptor are aligned in a linear geometry for SN2 nucleophilic transfer of the methyl group during catalysis. Differences in hydrogen bonding between the MeLys e-amino group and Rubisco LSMT and SET7/9 explain why Rubisco LSMT generates multiply methylated Lys, wheras SET7/9 generates only MeLys
UR  - https://drive.google.com/file/d/1oxmsE2R9GGrqt3eOZKSvmDd8NCTq5WwM/view?usp=drivesdk
ER  -