Bacterial growth in plant tissue culture media 

 -  Journal of Applied Bacteriology, 72(6), p.460-466, 1992 .
<br/><br/> When Murashige and Skoog's liquid plant medium was inoculated with 10 different bacterial species in the absence of plants only Bacillus subtilis showed significant growth. The numbers of Lactobacillus plantarum, Pseudomonas maltophilia, Erwinia carotovora and Staphylococcus saprophyticus decreased rapidly and were not detected at 28 d. Bacillus subtilis, Lact. plantarum, Ps. maltophilia, Erw. carotovora and Staph. saprophyticus grew and persisted in the same medium in the presence of Delphinium plants, while only Lact. plantarum and Erw. carotovora grew and persisted in the presence of Hemerocallis plants. Hemerocallis plants lowered the pH of media from 5.6 to about 3.9 while Delphinium plants increased it to about 5.9 within 7 d after subculturing on fresh media. The pH drop in Hemerocallis media is thought to prevent the growth and persistence of bacteria such as B. subtilis, Staph. saprophyticus and Ps. maltophilia, which were found to be more sensitive to low pH than Lact. plantarum and Erw. carotovora. Bacterial growth in the medium altered the pH, reduced the plant growth and/or resulted in plant death. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>BACTERIAL GROWTH<br/>BACTERIUM CULTURE<br/>CULTURE MEDIUM<br/>NONHUMAN<br/>PLANT