TY  - BOOK
AU  - Liu,S.
AU  - Wang,J.
AU  - Han,Z.
AU  - Gong,X.
AU  - Zhang,H.
AU  - Chai,J.
TI  - Molecular Mechanism for Fungal Cell Wall Recognition by Rice Chitin Receptor OsCEBiP
KW  - PLANT INNATE IMMUNITY
KW  - PERCEPTION
KW  - ARABIDOPSIS
KW  - PROTEINS
KW  - KINASE
KW  - CERK1
KW  - MOTIF
KW  - CEBIP
KW  - PEPTIDOGLYCAN
KW  - DIMERIZATION
N2  - Chitin is the major component of fungal cell wall and serves as a molecular pattern that can be recognized by the receptor OsCEBiP in rice, a lysine motif (LysM)receptor-like protein (RLP), to trigger immune responses. The molecular mechanisms underlying chitin recognition remain elusive. Here we report the crystal structures of the ectodomain of OsCEBiP (OsCEBiP-ECD)in free and chitin-bound forms. The structures reveal that OsCEBiP-ECD contains three tandem LysMs followed by a novel structure fold of cysteine-rich domain. The structures showed that chitin binding induces no striking conformational changes in OsCEBiP. Structural comparison among N-acetylglucosamine (NAG)oligomer-bound LysMs revealed a highly conserved recognition mechanism, which is expected to facilitate study of other LysM-containing proteins for their NAG binding. Modeling study showed that chitin induces OsCEBiP homodimerization in a "sliding mode". Our data provide insights into rice chitin receptor-mediated immunity triggered by fungal cell wall. © 2016 Elsevier Ltd
UR  - https://drive.google.com/file/d/1VY1fXyhrSlSF8CLI5ZeT7kMg296ybf3X/view?usp=drivesdk
ER  -