TY  - BOOK
AU  - Novák,Ondrej
AU  - Pencík,Ale
AU  - Blahousek,Ota
AU  - Ljung,Karin
TI  - Quantitative Auxin Metabolite Profiling Using Stable Isotope Dilution UHPLC-MS/MS
KW  - AUXIN
KW  - IAA
KW  - LC-MS/MS
KW  - METABOLITE PROFILING
KW  - SPE
N2  - Auxin, a plant hormone, is able to stimulate growth and to control developmental processes. To get a deeper level of understanding of how auxin activity is regulated in planta, we have developed a sensitive and selective mass spectrometry-based protocol for multiplex quantification of indole 3-acetic acid (IAA)as well as its precursors and degradation products. As part of the protocol development, we have also established a derivatization protocol for the IAA precursors IPyA and IAAld, compounds that are highly labile during sample extraction and purification. This article describes a standard isotope dilution method for quantitative profiling of the IAA metabolome in small amounts of plant tissues. © 2016 by John Wiley & Sons, Inc
UR  - https://drive.google.com/file/d/1gg4LE37umEQ0REGW86QL2zGjeDVJmyVz/view?usp=drivesdk
ER  -