Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion 

 -  Nature Biotechnology, 35(5), p.441-443, 2017 .
<br/><br/> We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID)for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs)in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>CROPS<br/>HERBICIDES<br/>NUCLEIC ACIDSPLANTS (BOTANY)<br/>WEED CONTROL