TY - BOOK AU - Mizukami,H. AU - Konoshima,M. AU - Tabata,M. TI - Effect of nutritional factors on shikonin derivative formation in Lithospermum callus cultures KW - 1,4-NAPHTHOQUINONE KW - BORAGINACEAE KW - LITHOSPERMUM ERYTHRORHIZON KW - NUTRITIONAL FACTORS KW - PLANT TISSUE CULTURE KW - REGULATION OF BIOSYNTHESIS KW - SHIKONIN DERIVATIVES N2 - Some nutritional factors affecting the biosynthesis of shikonin derivatives in callus cultures of Lithospermum erythrorhizon were examined. High sucrose concentrations increased the content of shikonin derivatives, but neither glucose nor fructose was effective for shikonin derivative formation. High concentrations of nitrogen sources inhibited or retarded shikonin derivative formation and streptomycin sulphate stimulated their biosynthesis. Addition of ascorbic acid increased the content of shikonin derivatives. Among some precursors tested only l-phenylalanine had a positive effect. At high concentrations, Ca2+ and Fe2+ inhibited the biosynthesis of shikonin derivatives UR - https://drive.google.com/file/d/154HwKtg7l8x1PWTkFc53BBTnbuWCDuIu/view?usp=drivesdk ER -