TY  - BOOK
AU  - Levy,L.W.
AU  - Regalado,E.
AU  - Navarrete,S.
AU  - Watkins,R.H.
TI  - Bixin and norbixin in human plasma: Determination and study of the absorption of a single-dose of annatto food color
KW  - BIXIN
KW  - CAROTENOIDS
KW  - HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
KW  - HUMAN PLASMA
KW  - INTESTINAL ADSORPTION
KW  - NORBIXIN
N2  - A procedure was developed for the detection and determination of bixin and norbixin in human plasma by reversed-phase HPLC with a sensitivity limit of 5 µg l-1. A group of seven volunteers ingested a single dose of 1 ml of a commercial Annatto Food Color (16 mg of cis-bixin in soybean oil). The presence of bixin (cis and trans)and norbixin (cis and trans)was demonstrated in the plasma at average levels of 11.6, 10.1, 2.8 and 0 µg l-1 of bixin and 48, 58, 53 and 29 µg l-1 of norbixin after 2, 4, 6 and 8 h, respectively. Considerable individual variations were observed. Complete plasma clearance generally occurred for bixin by 8 h and for norbixin by 24 h after ingestion of cis-bixin
UR  - https://drive.google.com/file/d/1UDHHsLN1SqfvGzilK0LSzWNjyBbMqLK2/view?usp=drivesdk
ER  -