Effective selection and regeneration of transgenic sugarcane plants using positive selection system 

 -  In Vitro Cellular & Developmental Biology-Plant, 51(1), p.52-61, 2015 .
<br/><br/> We compared the transformation frequency of a positive selection system, which employs an Escherichia coli-derived pmi (phosphomannose isomerase)gene as the selectable marker and mannose as the selective agent, to commonly used negative selection systems of bar (phosphinothricin acetyltransferase)/phosphinothricin (PPT), npt II (neomycin phosphotransferase)/G418 (geneticin), and hpt (hygromycin phosphotransferase)/Hygromycin. Transgenic sugarcane plants were obtained following biolistic transformation of callus with four different selectable marker genes. Under different selective agents, transformed callus was selectively proliferated on MS medium containing 13.57 然 of 2,4-D, regenerated on MS medium with 4.44 然 of BA, 9.29 然 of KT, and 0.89 然 of NAA, and rooted on half of MS medium with 13.38 然 of NAA and 0.44 然 of 6-BA. The results demonstrated that the transformation frequency of 4.2