TY  - BOOK
AU  - Scheidt,G.N.
AU  - Da Silva,A.L.L.
AU  - De Oliveira,Y.
AU  - Da Luz Costa,J.
AU  - Biasi,L.A.
AU  - Soccol,C.R.
TI  - In vitro growth of Melaleuca alternifolia Cheel in bioreactor of immersion by bubbles
KW  - MELALEUCA
KW  - MELALEUCA ALTERNIFOLIA
N2  - Development of new techniques for in vitro culture is necessary to optimize and reduce the production costs. The aim of this work was to introduce the bioreactor BIB® (Bioreactor of Immersion by Bubbles)and compare it with conventional culture and bioreactor RITA® (Recipient with Automatized Temporary Immersion)for In vitro growth of M. alternifolia plantlets. Plantlets with 1.0 cm height from the fifth In vitro subculture were used. Treatments were: (1)conventional culture (control)using 200 mL glass flasks covered with polypropylene lids; (2)bioreactor RITA® and (3)bioreactor BIB®. Continuous immersion and aeration was used in bioreactors. It was used liquid MS medium supplemented with 30 g.L-1 sucrose and 1 drop.200 mL-1 Tween 20® (i.e. detergent for bubbles formation). BIB® obtained best results for total fresh mass (0.39 g)and shoot fresh mass (0.27 g), fresh mass increment (3.1), total dry mass (0.036 g)and shoot dry mass (0.033 g)than RITA® and conventional culture. In conclusion, BIB® is more efficient to the biomass accumulation of M. alternifolia plantlets
UR  - https://drive.google.com/file/d/13UVRQ-HhZUa1uCANcXLkqAjn8blfnRdR/view?usp=drivesdk
ER  -