Crop Breeding Using CRISPR/Cas9 

 -  Crop Improvement Through Microbial Biotechnology, https://doi.org/10.1016/B978-0-444-63987-5.00023-2, p.451-464, 2018 .
<br/><br/> Genome editing can be achieved by inducing site-directed genome modification using artificial nucleases. The CRISPR/Cas9 system employs an RNA-guided nuclease that specifically recognizes and digests the target DNA of interest and has been utilized widely in genome editing in various organisms, including crops. Attempts have been made to optimize and improve CRISPR/Cas9 technology specifically for crop genome editing. Furthermore, new methods for introducing CRISPR/Cas9 tools into the cells of crop plants are also now being developed. CRISPR/Cas9 technology not only is a basic research tool but also has become one of the most useful molecular breeding tools currently available, and superior traits in various crop species have now been established. In this chapter, we describe the current status and perspectives of crop molecular breeding using CRISPR/Cas9. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>GENOME EDITING<br/>CRISPR/CAS9<br/>CROP BREEDING