TY  - BOOK
AU  - Kang,B.C.
AU  - Yun,J.Y.
AU  - Kim,S.T.
AU  - Shin,Y.
AU  - Ryu,J.
AU  - Choi,M.
AU  - Kim,J.S.
TI  - Precision genome engineering through adenine base editing in plants
KW  - GENOME ENGINEERING
N2  - The recent development of adenine base editors (ABEs)has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or mis-splicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide 'proof of concept' for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology
UR  - https://drive.google.com/file/d/1HCKqQb1qkb_EqsQQWmrXeC5zKDGyWCth/view?usp=drivesdk
ER  -