CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response 

 -  Nature Medicine, 24, p.927-930, 2018 .
<br/><br/> Here, we report that genome editing by CRISPR-Cas9 induces a p53-mediated DNA damage response and cell cycle arrest in immortalized human retinal pigment epithelial cells, leading to a selection against cells with a functional p53 pathway. Inhibition of p53 prevents the damage response and increases the rate of homologous recombination from a donor template. These results suggest that p53 inhibition may improve the efficiency of genome editing of untransformed cells and that p53 function should be monitored when developing cell-based therapies utilizing CRISPR-Cas9. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>CRISPR-CAS9