TY  - BOOK
AU  - Haapaniemi,E.
AU  - Botla,S.
AU  - Persson,J.
AU  - Schmierer,B.
AU  - Taipale,J.
TI  - CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response
KW  - CRISPR-CAS9
N2  - Here, we report that genome editing by CRISPR-Cas9 induces a p53-mediated DNA damage response and cell cycle arrest in immortalized human retinal pigment epithelial cells, leading to a selection against cells with a functional p53 pathway. Inhibition of p53 prevents the damage response and increases the rate of homologous recombination from a donor template. These results suggest that p53 inhibition may improve the efficiency of genome editing of untransformed cells and that p53 function should be monitored when developing cell-based therapies utilizing CRISPR-Cas9
UR  - https://drive.google.com/file/d/146tLCpYdLB1fxzYd9jMdjyZHMNQKpRrH/view?usp=drivesdk
ER  -