TY  - BOOK
AU  - Hallahan,D.L.
AU  - Lau,S.M.C.
AU  - Harder,P.A.
AU  - Smiley,D.W.
AU  - Dawson,G.W.
AU  - Pickett,J.A.
AU  - O'Keefe,D.P.
TI  - Cytochrome P-450-catalysed monoterpenoid oxidation in catmint (Nepeta racemosa)and avocado (Persea americana); evidence for related enzymes with different activities
KW  - CYTOCHROME P-450
KW  - PLANT ENZYME
KW  - SUBSTRATE SPECIFICITY
KW  - MONOTERPENOID
KW  - GENES
KW  - HYBRIDIZATION
KW  - (N. RACEMOSA)
KW  - (P. AMERICANA)
N2  - A cytochrome P-450 present in ripening avocado (Persea americana)fruit mesocarp (CYTP71A1)had previously been shown to metabolize the monoterpenoids nerol and geraniol (Hallahan et al. (1992)Plant Physiol. 98, 1290-1297). Using DNA encoding CYP71A1 as a hybridization probe, we have shown by Southern analysis that a related gene is present in the catmint, Nepeta racemosa. RNA blot analysis, together with Western analysis of catmint leaf polypeptides using avocado cyt P-450 antiserum, showed that a closely related gene is expressed in catmint leaves. Cytochrome P-450 in catmint microsomes catalysed the specific hydroxylation of nerol and geraniol at C-10, whereas avocado CYP71A1, in either avocado microsomes or heterologously expressed in yeast, catalysed 2,3- or 6,7-epoxidation of these substrates. These results suggest that orthologous genes of the CYP71 family are expressed in these two plant species, but catalyse dissimilar reactions with monoterpenoid substrates
UR  - https://drive.google.com/file/d/1A3M-A9XFSMREcTjDp2E0adgqHVjxQHRO/view?usp=drivesdk
ER  -