TY  - BOOK
AU  - Mishra,G.
AU  - Collings,D.A.
AU  - Altaner,C.M.
TI  - Physiological changes during heartwood formation young Eucalyptus bosistoana trees
KW  - AMYLOPLASTS
KW  - CONFOCAL MICROSCOPY
KW  - EXTRACTIVES
KW  - JUVENILE COREWOOD
KW  - NUCLEI
KW  - PARENCHYMA CELLS
KW  - RAY CELLS
KW  - TYLOSES
KW  - WOOD
N2  - Eucalyptus bosistoana F.Muell. is valued for its naturally durable heartwood. As part of an E. bosistoana breeding programme, we have tested the hypothesis that there is a prolonged transition from sapwood to heartwood in young trees, resulting in a wide transition zone. This needs to be considered when assessing trees for heartwood quantity and quality. Heartwood formation was investigated in radial profiles in cores from bark to bark of 6-year-old trees with conventional and confocal microscopy, and with a range of different staining techniques that visualised the physiological changes taking place in the parenchyma cells. Using immunolabelling with antibodies against histone proteins and ?-tubulin, histochemical staining using potassium iodide (I3-KI)and fluorescence emission spectral scanning, we demonstrated that in heartwood nuclei, microtubules, reserve materials (starch)and vacuoles were absent. The observations revealed that 6-year-old E. bosistoana trees contained heartwood. The loss of wáter conductivity by tyloses formation and the death of the parenchyma cells occurred in close proximity resulting in a transition zone of ~1 cm
UR  - https://drive.google.com/file/d/1PIKI5MQzqnx5Ms8YzWbmD1E44YBCX04U/view?usp=drivesdk
ER  -