CRISPR-Local: a local single-guide RNA (sgRNA)design tool for non-reference plant genomes 

 -  Bioinformatics, 35(14), p.2501-2503, 2018 .
<br/><br/> CRISPR-Local is a high-throughput local tool for designing single-guide RNAs (sgRNAs)in plants and other organisms that factors in genetic variation and is optimized to generate genome-wide sgRNAs. CRISPR-Local outperforms other sgRNA design tools in the following respects: (i)designing sgRNAs suitable for non-reference varieties; (ii)screening for sgRNAs that are capable of simultaneously targeting multiple genes; (iii)saving computational resources by avoiding repeated calculations from multiple submissions and (iv)running offline, with both command-line and graphical user interface modes and the ability to export multiple formats for further batch analysis or visualization. We have applied CRISPR-Local to 71 public plant genomes, using both CRISPR/Cas9 and CRISPR/cpf1 systems 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PLANT GENOMES