TY  - BOOK
AU  - Endo,M.
AU  - Mikami,M.
AU  - Endo,A.
AU  - Kaya,H.
AU  - Itoh,T.
AU  - Nishimasu,H.
AU  - Toki,S.
TI  - Genome editing in plants by engineered CRISPR-Cas9 recognizing NG PAM
KW  - CRISPR
N2  - Streptococcus pyogenes Cas9 (SpCas9)is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1)can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence
UR  - https://drive.google.com/file/d/1HHpkVe27XNeuVaBTw1SL-JVMZOv1C7DY/view?usp=drivesdk
ER  -