TY  - BOOK
AU  - Gan C.S.
AU  - Guo T.
AU  - Zhang H.
AU  - Kiang S.
TI  - A Comparative Study of Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC)versus SCX-IMAC-Based Methods for Phosphopeptide Isolation/Enrichment
KW  - PHOSPHOPEPTIDE ENRICHMENT
KW  - EGFR SIGNALING
KW  - A431 CARCINOMA CELLS
KW  - WAX
KW  - IMAC
KW  - ERLIC
KW  - SCX
N2  - Lectrostatic repulsion-hydrophilic interaction chromatography (ERLIC)has been introduced recently for phosphopeptide enrichment. Here we compared ERLIC with the well-established SCX-IMAC for identifying phosphopeptides in EGF-treated A431 cells. The ERLIC approach detected a higher number of phosphopeptides (17 311)than SCX-IMAC (4850), but it only detected 926 unique phosphopeptides compared to 1315 in SCX-IMAC. Only 12percent unique phosphopeptides were common to both pproaches, suggesting that more comprehensive phosphoproteomes could be generated by complementing SCXIMAC with ERLIC
UR  - https://drive.google.com/file/d/1zqD-aM99w6xHZq-HgQ0QAoITzMC24_3b/view?usp=drivesdk
ER  -