TY  - BOOK
AU  - Entsch,B.
AU  - Parker,C.W.
AU  - Letham,D.S.
TI  - An enzyme from lupin seeds forming alanine derivatives of cytokinins
KW  - LUPINUS LUTEUS
KW  - LEGUMINOSAE
KW  - CYTOKININ METABOLISM
KW  - ENZYME FORMING ALANYL CONJUGATES
KW  - PURIFICATION
N2  - A new enzyme, which catalyses the conversion of the cytokinin zeatin to the alanine conjugate lupinic acid, has been partly purified from developing lupin seed (Lupinus luteus). Paired-ion, reverse phase HPLC was adapted to analyse the enzyme reaction quantitatively. The enzyme used O-acetyl-l-serine as the source of the amino acid residue, and it interacted with substrates in a ping pong bi bi mechanism. A number of adenine derivatives served as substrates, but preference was shown for compounds with high cytokinin activity. The possible role of the enzyme, tentatively called B-(9-cytokinin)alanine synthase or lupinic acid synthase, in the regulation of hormone activity is discussed
UR  - https://drive.google.com/file/d/1QY244TKfvENgV9T7zbtYkGHGexcS87Ot/view?usp=drivesdk
ER  -