TY - BOOK AU - Strnad,M. AU - Peters,W. AU - Hanus,J. AU - Beck,E. TI - Ortho-topolin-9-glucoside, an aromatic cytokinin from Populus x canadensis cv Robusta leaves KW - POPULUS X CANADENSIS MOENCH CV ROBUST KW - POPLAR KW - SALICACEAE KW - AROMATIC CYTOKININ KW - ND O-HYDROXYBENZYLAMINO-9-O-?-D-GLUCOPYRANOSYLPURINE KW - ORTHO-TOPOLIN-9-GLUCOSIDE N2 - A new natural cytokinin was isolated from Populus x canadensis Moench cv Robusta leaves. Its structure was established as N6-(o-hydroxybenzylamino-9-O-?-d-glucopyranosylpurine (termed ortho-topolin-9-glucoside)by UV and mass spectrometry and confirmed by synthesis. This cytokinin exhibited comparatively low biological activity in three common bioassays. It was quantified in the leaf extract by combined high performance liquid chromatography-enzyme linked immunoassay. Amounting to 50 pmol g?1 fresh weight, ortho-topolin-9-glucoside was the dominant aromatic cytokinin in poplar leaves collected 2 hr after sunrise UR - https://drive.google.com/file/d/1jZnilEDH9jktooQ0Y3E2OHPrzU5-T1bl/view?usp=drivesdk ER -