Tandem Affinity Purification and Mass Spectrometry (TAP-MS)for the Analysis of Protein Complexes 

 -  Current Protocols in protein Science, 96, p. https://doi.org/10.1002/cpps.84, 2019 .
<br/><br/> Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP)primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure.