TY  - BOOK
AU  - Adelmant,G.
AU  - Garg,B.K.
AU  - Tavares,M.
AU  - Card,J.D.
AU  - Marto,J.A.
TI  - Tandem Affinity Purification and Mass Spectrometry (TAP-MS)for the Analysis of Protein Complexes
N2  - Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP)primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure
UR  - https://drive.google.com/file/d/1LgnnvgFzFv1Kpm0LFB9kXTYFjoc6X8Ot/view?usp=drivesdk
ER  -