Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13.
- The CRISPR Journal,, 2(3), p.165-171, 2019 .
Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identi?cation. Here, we use a modi?ed version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. SHERLOCK is rapid (*15min), quantitative, and portable, and can process crude soybean extracts as input material for minimal nucleic acid sample preparation. This ?eld-ready SHERLOCK platform with color-based lateral ?ow readout can be applied for detection and quantitation of genes in a range of agricultural applications.