TY  - BOOK
AU  - Abudayyeh,O.O.
AU  - Gootenberg,J.S.
AU  - Kellner,M.J.
AU  - Zhang,F.
TI  - Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13
KW  - CRISPR-CAS13
N2  - Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identi?cation. Here, we use a modi?ed version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. SHERLOCK is rapid (*15min), quantitative, and portable, and can process crude soybean extracts as input material for minimal nucleic acid sample preparation. This ?eld-ready SHERLOCK platform with color-based lateral ?ow readout can be applied for detection and quantitation of genes in a range of agricultural applications
UR  - https://drive.google.com/file/d/1EWsIVkxerCapNNNXM0Qwbo45qPldg0eb/view?usp=drivesdk
ER  -