Disruption of protease genes in microbes for production of heterologous proteins. 

 -  In New and Future Developments in Microbial Biotechnology and BioEngineering, https://doi.org/10.1016/B978-0-444-63503-7.00003-6, p.35-75, 2019 .
<br/><br/> Proteins, known as the building blocks of life, are synthesized by all forms of life as part of their natural metabolism. Certain proteins, including enzymes, act as biocatalysts and enhance the rate of metabolic reactions. With the advancements of genetic and protein engineering, the commercial production of proteins has increased and recombinant proteins are benefitting the major sectors of the biopharmaceutical, enzyme, and agricultural industries. Microbes serve as the best, most attractive and cost-effective cell factories for the production of various proteins. Various microbes, including Agrobacterium, Aspergillus, Bacillus, Cellulosimicrobium, Chaetomium, Chloroflexus, Dinoroseobacter, Enterococcus, Escherichia, Fusarium, Kluyveromyces, Lactobacillus, Penicillium, Pichia, Pseudomonas, Rhodococcus, Saccharomyces, and Streptomyces are being utilized for heterologous protein production and each one has its own advantages and disadvantages. The major problem that interferes with heterologous protein production includes the proteolytic degradation of the secreted protein by the host proteases and to overcome this problem various strategies, including disruption of the interfering protease genes, classical mutagenesis to construct strains secreting low levels of proteases, and constructing protease-deficient strains which further enhance productivity. These techniques could not completely solve the problems, but have helped to deal with proteolytic degradation to a great extent. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>DISRUPTANT<br/>HETEROLOGOUS PROTEIN PRODUCTION<br/>METABOLIC ENGINEERING<br/>PROTEASE<br/>SYSTEMS BIOTECHNOLOGY.