TY  - BOOK
AU  - Sanchez-Gonzalez,N.
AU  - Jaime-Fonseca,M.R.
AU  - San Martin-Martinez,E.
AU  - Zepeda,L.G.
TI  - Extraction, stability, and separation of betalains from Opuntia joconostle cv. using response surface methodology
KW  - OPUNTIA
KW  - XOCONOSTLE
KW  - SOLVENT EXTRACTION
KW  - STABILITY
KW  - SEPARATION
KW  - HPLC
KW  - NATURAL COLORANTS
N2  - Betalains were extracted and analyzed from Opuntia joconostle (the prickly pear known as xoconostle in Mexico). For the extraction, two solvent systems were used, methanol/water and ethanol/water. A three-variable Box-Behnken statistical design was used for extraction: solvent concentration (0-80 per cent, v/v), temperature (5-30 °C), and treatment time (10-30 min). The extraction and stability of betalains from xoconostle were studied using response surface methodology (RSM). Techniques such as UV-vis, column chromatography, and HPLC were employed for the separation and analysis of the main pigments present in the extracts. Maximum pigment concentration (92 mg/100 g of fruit)was obtained at a temperature of 15 °C and a time of 10 min for methanol/water (20:80), whereas maximum stability of the pigment was observed at pH 5 and a temperature of 25 °C. HPLC chromatograms showed the main betalains of the xoconostle characterized were betalain, betanidin, and isobetalain
UR  - https://drive.google.com/file/d/1jUPE_8tO9TgRpXL96FE1ky4IGx0srJ8z/view?usp=drivesdk
ER  -