TY  - BOOK
AU  - Bhushan,K.
TI  - Evolution and molecular mechanism of CRISPR/Cas9 systems
KW  - BACTERIA
KW  - CASCADE
KW  - CRISPR/CAS SYSTEMS
KW  - DCAS9
KW  - GENOME EDITING
KW  - RNA
N2  - CRISPR/Cas module is an RNA-guided adaptive immune system, provides sequence-specific immunity against phages and (conjugative)plasmids. CRISPR/Cas system is basically categorized into three types: (type I, II and III), on the basis of effector complexes. CRISPR/Cas9 system is repercussion of evolutionary everlasting arm race between bacteria and phages. Molecular mechanism underlying the CRISPR system entails three distinct mechanistic stages: spacer acquisition, crRNA biogenesis and interference. CRISPR/Cas9 system composed of a combination of Cas9 (an endonuclease)and a customizable single guide RNA (sgRNA), the later directs the Cas9 for recognition and degradation of invading DNA. Furthermore, Cas9 and their ilk proteins with unique properties have opened a plethora of options including DNA/RNA genome targeting, transcription/translation regulation and empower researchers to edit genes with highly precision and speed
UR  - https://drive.google.com/file/d/1OYdwUhuslep28rATaBWT__U21w7iUh83/view?usp=drivesdk
ER  -