TY  - BOOK
AU  - Lin,Q.
AU  - Zong,Y.
AU  - Xue,C.
AU  - Wang,S.
AU  - Jin,S.
AU  - Zhu,Z.
AU  - Liu,D.R.
TI  - Prime genome editing in rice and wheat
KW  - GENOME
N2  - Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8 percent
UR  - https://drive.google.com/file/d/1msMbIAuX2y4DPctUAUT28VMardk_1cbm/view?usp=drivesdk
ER  -