TY - BOOK AU - Fu,Y.F. AU - Zhang,Z.W. AU - Yang,X.Y. AU - Wang,C.Q. AU - Lan,T. AU - Tang,X.Y. AU - Yuan,S. TI - Nitrate reductase is a key enzyme responsible for nitrogen-regulated auxin accumulation in Arabidopsis roots KW - NITRATE REDUCTASE KW - NITRATE TRANSPORTER NRT1.1 KW - NITRATE SIGNALING KW - ROOT ARCHITECTURE REMODELING KW - AUXIN BIOSYNTHESIS AND TRANSPORT N2 - Nitrate reductase (NR)is one of the key enzymes for plant nitrogen assimilation and root architecture remodeling. However, crosstalk between NR-mediated signaling and auxin-mediated root development in nitrogen-status responses has not been investigated in details before. In this study, root phenotype and auxin distribution in nia1/nia2 (nitrate reductase)double mutant and chl1-5 (nitrate transporter NRT1.1)mutant under different nitrogen availabilities were compared. The nia1/nia2 mutant showed very low expression levels of auxin biosynthetic/signaling genes and was insensitive to nitrogen changes. While the chl1-5 mutant showed a high NR activity with a high level of auxin in the meristematic zone and a weaker response to nitrogen changes, when compared with the wild-type plants. We firstly found that NR activity was roughly positive-correlated with the root auxin level, and there is a crosstalk between nitrate signaling and auxin signaling. The putative signaling pathways downstream of NR have been discussed UR - https://drive.google.com/file/d/1ALQzcnGE2KtUTmcyZuIp1CkF86z0m45T/view?usp=drivesdk ER -