TY  - BOOK
AU  - Qiu,K.
AU  - Chen,B.
AU  - Nie,W.
AU  - Zhou,X.
AU  - Feng,W.
AU  - Wang,W.
AU  - He,C.
TI  - Electrophoretic deposition of dexamethasone-loaded mesoporous silica nanoparticles onto poly (L-lactic acid)/poly (?-caprolactone)composite scaffold for bone tissue engineering
KW  - ELECTROPHORETIC DEPOSITION
KW  - MESOPOROUS SILICA NANOPARTICLES (MSNS)
KW  - DEXAMETHASONE
KW  - POLY(L-LACTIC ACID)/POLY(??CAPROLACTONE)SCAFFOLD
KW  - BONE TISSUE ENGINEERING
N2  - The incorporation of microcarriers as drug delivery vehicles into polymeric scaffold for bone regeneration has aroused increasing interest. In this study, the aminated mesoporous silica nanoparticles (MSNs-NH2)were prepared and used as microcarriers for dexamethasone (DEX)loading. Poly(L-lactic acid)/poly(?-caprolactone)(PLLA/PCL)nanofibrous scaffold was fabricated via thermally induced phase separation (TIPS)and served as template, onto which the drug-loaded MSNs-NH2 nanoparticles were deposited by electrophoretic deposition (EPD). The physicochemical and release properties of the prepared scaffolds (DEX@MSNs-NH2/PLLA/PCL)were examined, and their osteogenic activities were also evaluated through in vitro and in vivo studies. The release of DEX from the scaffolds revealed an initial rapid release followed by a slower and sustained one. The in vitro results indicated that the DEX@MSNs-NH2/PLLA/PCL scaffold exhibited good biocompatibility to rat bone marrow-derived mesenchymal stem cells (BMSCs). Also, BMSCs cultured on the DEX@MSNs-NH2/PLLA/PCL scaffold exhibited a higher degree of osteogenic differentiation than those cultured on PLLA/PCL and MSNs-NH2/PLLA/PCL scaffolds, in terms of alkaline phosphatase (ALP)activity, mineralized matrix formation, and osteocalcin (OCN)expression. Furthermore, the in vivo results in a calvarial defect model of Sprague-Dawley (SD)rats demonstrated that the DEX@MSNs-NH2/PLLA/PCL scaffold could significantly promote calvarial defect healing compared with the PLLA/PCL scaffold. Thus, the EPD technique provides a convenient way to incorporate osteogenic agents-containing microcarriers to polymer scaffold, and thus, prepared composite scaffold could be a potential candidate for bone tissue engineering application due to its capacity for delivery of osteogenic agents
UR  - https://drive.google.com/file/d/1wkqMftEPgyf5RAFYqnKKN4Kvr-9GWf9f/view?usp=drivesdk
ER  -