TY  - BOOK
AU  - Mi,J.
AU  - Liew,K.X.
AU  - Al-Babili,S.
TI  - Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry Analysis of Carotenoid-Derived Hormones and Apocarotenoids in Plants
KW  - ABSCISIC ACID
KW  - APOCAROTENOIDS
KW  - RICE ROOT
KW  - SOLID-PHASE EXTRACTION
KW  - STRIGOLACTONES
KW  - ULTRASOUND-ASSISTED EXTRACTION
KW  - ULTRAHIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
N2  - Carotenoid oxidative cleavage products, apocarotenoids (APOs), are a class of important plant secondary metabolites, which include phytohormones abscisic acid (ABA)and strigolactones (SLs), and growth regulators and signaling molecules such as ?-cyclocitral, zaxinone, anchorene, ?-apo-11-carotenoids, and retinal. Qualitative and quantitative analysis of these bioactive compounds is crucial for understanding their metabolism and may also enable discovering further regulatory APOs. The state-of-the-art mass spectrometry (MS)technology has advanced the detection of plant APOs; however, it is still challenging to perform an accurate analysis of the low-level phytohormones ABA and SL and the structurally diverse APOs from complex plant matrices. Here, we describe ultrahigh-performance liquid chromatography-MS (UHPLC-MS)methods to determine carotenoid-derived hormones and APOs from plants by integrating ultrasound-assisted extraction and solid-phase extraction. These assays enable an accurate quantification of carotenoid-derived hormones and APOs from plant tissues by using an UHPLC hybrid quadrupole-Orbitrap mass spectrometer
UR  - https://drive.google.com/file/d/1iXfIAnefx2dJwbCOYnGtcRpKEsDw2dbh/view?usp=drivesdk
ER  -