Four new phenolic compounds from the tender leaves of Eucommia ulmoides Oliv. and their anti-inflammatory activities 

 -  PhytoChemistry Letters, 44, p.173-177, 2021 .
<br/><br/> Four new phenolic compounds, 2-propanoic acid (1), 2-propanoic acid methyl (2), (±)3-propanoic acid methyl (3), and (±)3-propanoic acid (4), together with five known compounds, methyl 3-(3,4-dihydroxyphenyl)-propanoate (5), 3,4-dihydroxybenzenepropionic acid (6), caffeic acid (7), protocatechuic acid (8), and 1,3-trans-5,6-dihydroxy-3-(3? ,4? -dihydroxyphenyl)indan-1-methyl (9)were isolated from the tender leaves of Eucommia ulmoides Oliv. Compound 9 was isolated from Eucommiaceae for the first time. The structures of the new compounds were elucidated from the spectra of 1D NMR and 2D NMR, and MS data. Compounds 1-9 were tested to determine their inhibitory effect on LPS-induced NO production in macrophage RAW264.7 cells. Modest inhibitory activities were observed for compounds 2-5 and 9, with IC50 values ranging from 8.3 to 31.0 ?M. This research enriches the current chemical information regarding the leaves of Eucommia ulmoides Oliv. and provides a reference for further research on its anti-inflammatory effects. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>TENDER LEAVES OF EUCOMMIA ULMOIDES<br/>PHENOLICS<br/>ANTI-INFLAMMATORY ACTIVITY