TY  - BOOK
AU  - Yinusa,I.
AU  - George,N.I.
AU  - Ayo,R.G.
AU  - Rufai,Y.
TI  - Vacuum liquid chromatographic isolation and characterization of beta-sitosterol from the bark of Sarcocephalus latifolius (Smith Bruce)
KW  - SARCOCEPHALUS
KW  - ISOLATION
KW  - METHANOL
KW  - CHROMATOGRAPHY
KW  - SPECTROSCOPIC
KW  - _-SITOSTEROL
N2  - The methanol fraction of the stem bark of Sarcocephalus Latifolius (Smith Bruce)was subjected to vacuum liquid chromatography (VLC)isolation method using an isocratic solvent of petroleum ether: ethyl acetate at a ratio of 1:9 by collecting 20ml each and 45 fractions were collected. Based on their Thin Layer Chromatographic (TLC)analysis, fraction 6, 7, 8 and 9 were combined to give SB-C which was further subjected to VLC and eluted isocratically using petroleum ether: ethyl acetate (1:9)to obtain SB-C-1 to SB-C-8 (8 Fraction). Fraction 5 shows a prominent single yellow spot that was cleaned further by Preparative Thin Layer Chromatography (PTLC)to obtain SB-C1. The structure of the compound was established by spectroscopic studies as well as by comparison with existing data. Based on these SB-C1 was proposed to be _-Sitosterol
UR  - https://drive.google.com/file/d/1Z0iKERIYVrIZ8o-RQFvn3NKd0WBQoASq/view?usp=drivesdk
ER  -