TY  - BOOK
AU  - Steliopoulos,P.
AU  - Wüst,M.
AU  - Adam,K.P.
AU  - Mosandl,A.
TI  - Biosynthesis of the sesquiterpene germacrene D in Solidago canadensis: 13C and 2H labeling studies
KW  - SOLIDAGO CANADENSIS
KW  - ASTERACEAE
KW  - BIOSYNTHESIS
KW  - METHYLERYTHRITOL PHOSPHATE PATHWAY
KW  - SESQUITERPENES
KW  - GERMACRENE D
KW  - LABELING STUDIES
N2  - The biogenetic origin of the isoprenoid building blocks of the sesquiterpene germacrene D was studied in Solidago canadensis. Feeding experiments were carried out with 1-[5,5-D(2)]deoxy-D-xylulose-5-phosphate (D(2)-DOXP), [5-13C]mevalonolactone (13C-MVL)and [1-13C]-D-glucose. The hydrodistillate of a cut shoot fed with D(2)-DOXP was investigated by enantio-MDGC-MS and the volatile fraction of a shoot supplied with 13C-MVL was examined by GC-C-IRMS. The incorporation of [1-13C]-D-glucose was analyzed by quantitative 13C NMR spectroscopy after isolation of germacrene D from the essential oil. Our labeling studies revealed that the biosynthesis of the C-15 skeleton of sesquiterpene germacrene D in Solidago canadensis proceeds predominantly via the methylerythritol phosphate pathway
UR  - https://drive.google.com/file/d/1klXLYLlxt5Zujg9B59xfVqxJLv5HMvEv/view?usp=drivesdk
ER  -