Improved DPPH Determination for Antioxidant Activity Spectrophotometric Assay 

 -  Chemical Papers, 61(3), p.214-216, 2007 .
<br/><br/> An improved procedure for determination of the residual DPPH (1,1-diphenyl-2-picrylhydrazyl)free radical concentration was proposed taking into account the absorbance of both DPPH free radicals and DPPH nonradical (1,1-diphenyl-2-picrylhydrazine)stable form. The calculated residual DPPH free radical concentrations were compared with those obtained from a calibration curve and variation coefficients below 10 percent were found. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>DPPH DETERMINATION<br/>ANTIOXIDANT ACTIVITY<br/>SPECTROPHOTOMETRIC ASSAY<br/>FREE RADICALS