TY  - BOOK
AU  - Ham,B.K.
AU  - Wang,X.
AU  - Toscano-Morales,R.
AU  - Lin,J.
AU  - Lucas,W.J.
TI  - Plasmodesmal endoplasmic reticulum proteins regulate intercellular trafficking of cucumber mosaic virus in Arabidopsis
KW  - PLASMODESMATA (PD)
KW  - NON-CELL AUTONOMOUS PROTEIN (NCAP)
KW  - ER-PROTEINS
KW  - VIRAL MOVEMENT PROTEIN
KW  - VIRUS INFECTION
KW  - CUCUMBER MOSAIC VIRUS (CMV)
N2  - Plasmodesmata (PD)are plasma membrane-lined cytoplasmic nanochannels that mediate cell-to-cell communication across the cell wall. A range of proteins are embedded in the PD plasma membrane and endoplasmic reticulum (ER), and function in regulating PD-mediated symplasmic trafficking. However, knowledge of the nature and function of the ER-embedded proteins in the intercellular movement of non-cell-autonomous proteins is limited. Here, we report the functional characterization of two ER luminal proteins, AtBiP1/2, and two ER integral membrane proteins, AtERdj2A/B, which are located within the PD. These PD proteins were identified as interacting proteins with cucumber mosaic virus (CMV)movement protein (MP)in co-immunoprecipitation studies using an Arabidopsis-derived plasmodesmal-enriched cell wall protein preparation (PECP). The AtBiP1/2 PD location was confirmed by TEM-based immunolocalization, and their AtBiP1/2 signal peptides (SPs)function in PD targeting. In vitro/in vivo pull-down assays revealed the association between AtBiP1/2 and CMV MP, mediated by AtERdj2A, through the formation of an AtBiP1/2-AtERdj2-CMV MP complex within PD. The role of this complex in CMV infection was established, as systemic infection was retarded in bip1/bip2w and erdj2b mutants. Our findings provide a model for a mechanism by which the CMV MP mediates cell-to-cell trafficking of its viral ribonucleoprotein complex
UR  - https://drive.google.com/file/d/1jvDBJqREqVbd4xowcIzvgtahdMbwNAia/view?usp=drivesdk
ER  -