TY  - BOOK
AU  - Van Den Engh,G.
AU  - Sachs,R.
AU  - Trask,B.J.
TI  - Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model
N2  - The folding of chromatin in interphase cell nuclei was studied by fluorescent in situ hybridization with pairs of unique DNA sequence probes. The sites of DNA sequences separated by 100 to 2000 kilobase pairs (kbp)are distributed in interphase chromatin according to a random walk model. This model provides the basis for calculating the spacing of sequences along the linear DNA molecule from interphase distance measurements. An interphase mapping strategy based on this model was tested with 13 probes from a 4-megabase pair (Mbp)region of chromosome 4 containing the Huntington disease locus. The results confirmed the locations of the probes and showed that the remaining gap in the published maps of this region is negligible in size. Interphase distance measurements should facilitate construction of chromosome maps with an average marker density of one per 100 kbp, approximately ten times greater than that achieved by hybridization to metaphase chromosomes
UR  - https://drive.google.com/file/d/1dGt6NaR-OG7GjiZkIq_RLfLsBzSJ32Hq/view?usp=drivesdk
ER  -