TY  - BOOK
AU  - Mahajan,V.
AU  - Chouhan,R.
AU  - Jamwal,V.L.
AU  - Kapoor,N.
AU  - Gandhi,S.G.
TI  - A wound inducible chalcone synthase gene from Dysoxylum gotadhora (DbCHS)regulates flavonoid biosynthesis
KW  - ANTIMICROBIAL ACTIVITY
KW  - CHALCONE SYNTHASE
KW  - DYSOXYLUM BINECTARIFERUM
KW  - DYSOXYLUM GOTADHORA
KW  - FLAVONOIDS
KW  - SECONDARY METABOLITES
N2  - Chalcone synthase (CHS)is a type III polyketide synthase and a key enzyme of the phenylpropanoid pathway that generates precursors for flavonoid biosynthesis. The tree species D. gotadhora is known for having an abundance of rohitukine, which has anti-inflammatory and immune-modulating effects. In this study, we used the leaves of D. gotadhora to clone CHS gene (DbCHS). The 1188-bp open reading frame (ORF)was part of the 1373-bp full-length DbCHS clone. Compared to other parts of the plant, DbCHS is expressed more in the leaves and fruits. This is linked to anti-microbial action against a panel of microbes in these tissues. The leaves and seeds extracts inhibit Bacillus subtilis, Streptococcus pyogenes, Bacillus cereus, and Candida albicans. When a plant is hurt, it leaves its tissues open to attack by microbes. To protect themselves, plants often make chemicals that kill microbes. We found that wounding had a big effect on the production of DbCHS. Based on these tests and the results of phylogenetic analysis and molecular docking, we believe that DbCHS is a wound-inducible enzyme that is needed to make flavonoids, which may give the plant antimicrobial properties
UR  - https://drive.google.com/file/d/1hhdkR0YnUN3Ai8SSq6zAKHM29BTAPkFU/view?usp=drivesdk
ER  -