Regeneration of a transgenic woody legume (Robinia pseudoacacia L., black locust)and morphological alterations induced by Agrobacterium rhizogenes-mediated transformation 

 -  Plant Science, 88(2), p.149-157, 1993 .
<br/><br/> A repeatable and fast transformation-regeneration system has been developed for a woody legume, black locust (Robinia pseudoacacia L.). Hairy roots were produced from hypocotyl segments inoculated with Agrobacterium rhizogenes R1601, a super-virulent strain with eukaryotic kanamycin resistance gene, in less than 1 week and resulted in subsequent shoot regeneration within 4 weeks. Transgenic black locust trees were established in soil 12 weeks after inoculation with the A. rhizogenes strain. The hairy root phenotype found in regenerated plants, which has been characterized as having wrinkled leaves and abundant root development, became evident after 3 months of growth. Southern blot analysis clearly showed multiple integration of T-DNA (transferred DNA)and the presence of neomycin phosphotransferase (NPTII)gene sequences in the transformed black locust genome. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>AGROBACTERIUM<br/>BLACK LOCUST<br/>HAIRY ROOT<br/>LEGUME<br/>TRANSFORMATION