Relationship between somatic embryogenesis and endogenous hormones of Cinnamomum camphora L.
- Plant Cell, Tissue and Organ Culture, 156(2), p.1-9, 2024 .
The relationship between different types of explants and the content of endogenous hormones during somatic embryogenesis was studied by high-performance liquid chromatography-mass spectrometry (HPLC-MS)in Cinnamomum camphora, providing a theoretical basis for understanding the mechanism underlying the development of somatic embryos in the process of somatic embryogenesis. Embryogenic callus (EC), non-embryogenic callus (NEC), and somatic embryos (SE)were used as research materials to measure and analyze the activity of metabolic-related enzymes. The results showed that the activity of superoxide dismutase (SOD)and peroxidase (POD)reached the highest in the EC and SE, which could promote and induce the development of embryonic cells. Lower activity of malondialdehyde (MDA)was observed in EC and SE, indicating that they had stronger stress resistance. Lower levels of catalase (CAT)metabolism might promote differentiation of somatic embryos. The contents of indole-3-acetic acid (IAA)and abscisic acid (ABA)were higher in all three types of materials, and the ratios of ABA/IAA, ABA/gibberellins (GA3), and IAA/zeatin (ZT)reached the highest values in SE followed by EC, indicating that higher ratios of ABA/IAA, ABA/GA3, and IAA/ZT were beneficial to somatic embryogenesis.