TY  - BOOK
AU  - Ovbude S.T.
AU  - Sharmeen S.
AU  - Kyei I.
AU  - Olupathage H.
AU  - Jones J.
AU  - Bell R.J.
AU  - Powers R.
AU  - Hage D.S.
TI  - Applications of chromatographic methods in metabolomics: A review
T2  - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 1239, 124124, 2024, DOI: 10.1016/j.jchromb.2024.124124
KW  - GAS CHROMATOGRAPHY
KW  - LIQUID CHROMATOGRAPHY
KW  - MASS SPECTROMETRY
KW  - METABOLOMICS
KW  - NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
KW  - SUPERCRITICAL FLUID CHROMATOGRAPHY
N2  - Chromatography is a robust and reliable separation method that can use various stationary phases to separate complex mixtures commonly seen in metabolomics. This review examines the types of chromatography and stationary phases that have been used in targeted or untargeted metabolomics with methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. General considerations for sample pretreatment and separations in metabolomics are considered, along with the various supports and separation formats for chromatography that have been used in such work. The types of liquid chromatography (LC) that have been most extensively used in metabolomics will be examined, such as reversed-phase liquid chromatography and hydrophilic liquid interaction chromatography. In addition, other forms of LC that have been used in more limited applications for metabolomics (e.g., ion-exchange, size-exclusion, and affinity methods) will be discussed to illustrate how these techniques may be utilized for new and future research in this field. Multidimensional LC methods are also discussed, as well as the use of gas chromatography and supercritical fluid chromatography in metabolomics. In addition, the roles of chromatography in NMR- vs. MS-based metabolomics are considered. Applications are given within the field of metabolomics for each type of chromatography, along with potential advantages or limitations of these separation methods. © 2024
UR  - https://drive.google.com/file/d/1op-BHjRAVfyFS9NKdP3D6cHoniU_m6ns/view?usp=drivesdk
ER  -