Visualizing calcium-dependent signaling networks in plants 

 - Trends in Plant Science. 29(2), 117-119, 2024, DOI: 10.1016/j.tplants.2023.11.004 .
<br/><br/> Calcium-dependent protein kinases (CDPKs) are a multigene protein kinase family that have key regulatory roles in plants. However, imaging CDPK signals in plant cells remains challenging. The recently developed genetically encoded CDPK-Förster resonance energy transfer (FRET) reporter developed by Liese et al. allows visualization of calcium (Ca2+)-dependent conformational changes during activation or inactivation of CDPKs, providing a powerful tool for real-time monitoring of calcium decoding in plants. © 2023 Elsevier Ltd