TY  - BOOK
AU  - Sarkar,J.
AU  - Jyoti,T.P.
AU  - Sahana,S.
AU  - Bhattacharya,A.
AU  - Chandel,S.
AU  - Singh,R.
TI  - CRISPR-Cas13d in plant biology: an insight
T2  - Plant Biotechnology Reports. 18(3), 301-311, 2024, DOI: https://doi.org/10.1007/s11816-024-00893-6
KW  - CRISPR
KW  - CAS13D
KW  - PLANT BIOLOGY
KW  - RNA EDITING
N2  - Plants are among the many creatures that have benefited from the widespread application of the CRISPR-associated Cas system as a genome-editing tool for investigating gene function, identifying disease, and enhancing agricultural yields. Although the CRISPR/Cas systems for DNA editing are widely employed, post-transcriptional manipulation of RNA remains difficult despite the prevalence of Cas9. Type VI CRISPR/Cas systems, which were recently found, allow for precise RNA editing without permanently affecting the genome. Cas13d has been put to good use in RNA-related studies across a wide range of RNA knock-down, and RNA detection without affecting DNA. Regulation of cas13d specificity and activity helps to avoid the off-target effects and immune responses in plants. Cas13d as highly efficient RNA-targeting tools for the virus resistance, gene function studies, disease diagnostics, and crop improvement in plants. However, CRISPR/Cas13d applications in plant RNA biology are just getting started. This article discusses how RNA editing tools derived from the CRISPR/Cas13d system are currently being used and where they may be used in the future for plant research
UR  - https://drive.google.com/file/d/1KQuRqIJYcMdvsGew2mZ1gg8vrXQAnfg3/view?usp=drivesdk
ER  -