The Inoue method for preparation and transformation of competent Escherichia coli:"Ultracompetent" cells 

 - Cold Spring Harbor Protocol, 6, p.pdb-prot101196, 2020 .
<br/><br/> Artículo 
<br/><br/> This protocol differs from other transformation procedures in that the bacterial culture is grown at 18 C rather than the conventional 37 C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Under standard laboratory conditions, efficiencies of 1× 10 8 to 3× 10 8 transformed colonies/µg of plasmid DNA are typical. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>TRANSFORMATION<br/>COMPETENT ESCHERICHIA COLI