TY  - BOOK
AU  - Green,M.R.
AU  - Sambrook,J.
TI  - The Inoue method for preparation and transformation of competent Escherichia coli:"Ultracompetent" cells
T2  - Cold Spring Harbor Protocol, 6, p.pdb-prot101196, 2020
KW  - TRANSFORMATION
KW  - COMPETENT ESCHERICHIA COLI
N1  - Artículo
N2  - This protocol differs from other transformation procedures in that the bacterial culture is grown at 18 C rather than the conventional 37 C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Under standard laboratory conditions, efficiencies of 1× 10 8 to 3× 10 8 transformed colonies/µg of plasmid DNA are typical
UR  - https://drive.google.com/file/d/1iANnzpPpF1gkFq9epIYnTLA1XNtna6zw/view?usp=drive_link
ER  -